JoVE Logo
Faculty Resource Center

Sign In

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

DOI :

10.3791/54583-v

12:28 min

October 15th, 2016

October 15th, 2016

11,028 Views

1Fischell Department of Bioengineering, University of Maryland, 2Department of Chemical and Biomolecular Engineering, University of Maryland

We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane.

Tags

Bacterial Inner membrane Display

-- Views

Related Videos

article

Laboratory Scale Production and Purification of a Therapeutic Antibody

article

Analysis of Histone Antibody Specificity with Peptide Microarrays

article

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

article

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans

article

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

article

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

article

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

article

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

article

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

article

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved