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Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

DOI :

10.3791/61871-v

September 22nd, 2020

September 22nd, 2020

3,244 Views

1Graduate School of Medicine, Ehime University, 2Proteo-Science Center, Ehime University

This protocol describes an efficient cell-free method for production of high-quality proteoliposome by bilayer-dialysis method using wheat cell-free system and liposomes. This method provides suitable means for functional analysis of membrane proteins, drug targets screening, and antibody development.

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