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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

DOI :

10.3791/59809-v

0:09 min

August 22nd, 2019

August 22nd, 2019

10,063 Views

1World Without Disease Accelerator, Janssen Pharmaceutical Companies of Johnson and Johnson, 2Janssen Prevention Center, Janssen Pharmaceutical Companies of Johnson and Johnson

The BSelex method to identify and recover individual antigen-specific antibodies from human peripheral blood mononuclear cells combines flow cytometry with single cell PCR and cloning.

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Single cell Screening Method

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