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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

DOI :

10.3791/50685-v

February 16th, 2014

February 16th, 2014

33,312 Views

1Department of Horticulture, University of Kentucky

Phage display is a powerful technique to capture proteins or protein moieties that interact with an immobilized molecule of interest. Once a decision of the type of phage cDNA library to create and screen has been made, the protocol described here permits efficient affinity selection leading to identification of interactors.

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