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G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

DOI :

10.3791/54696-v

September 10th, 2016

September 10th, 2016

9,416 Views

1Genetics, Cell Biology, and Development, University of Minnesota, 2Department of Cell and Developmental Biology, University of Michigan

Simple methods to detect the selective activation of G proteins by G protein-coupled receptors remain an outstanding challenge in cell signaling. Here, Fӧrster resonance energy transfer (FRET) biosensors have been developed by pairwise tethering a GPCR to G protein peptides to probe conformational changes at controlled concentrations in live cells.

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