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A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors

DOI :

10.3791/64505-v

December 2nd, 2022

December 2nd, 2022

1,763 Views

1Department of Entomology, Texas A&M University, 2Department of Biochemistry and Biophysics, Texas A&M University

In this work, a high-throughput, intracellular calcium fluorescence assay for 384-well plates to screen small molecule libraries on recombinant G protein-coupled receptors (GPCRs) is described. The target, the kinin receptor from the cattle fever tick, Rhipicephalus microplus, is expressed in CHO-K1 cells. This assay identifies agonists and antagonists using the same cells in one "dual-addition" assay.

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Dual addition Calcium Fluorescence Assay

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