JoVE Logo
Faculty Resource Center

Sign In

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

DOI :

10.3791/54799-v

March 3rd, 2017

March 3rd, 2017

10,413 Views

1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University

Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.

-- Views

Related Videos

article

Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein

article

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

article

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

article

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

article

In vitro Uncoating of HIV-1 Cores

article

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

article

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

article

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

article

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

article

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved