Results: SILAC Is an Effective Method for Analyzing the Effects of HIV-1 Infection on Host Exosomal Proteomes
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Conclusion
Transkript
The overall goal of this procedure is to characterize the exosomal proteome from HIV-1 infected cells. This method can help answer the key question in the exosome biology field such as the effect of external stresses on the composition of the exos
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Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.