Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

10.8K Views

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10:13 min

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December 12th, 2016

DOI :

10.3791/55002-v

December 12th, 2016

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Jing Men¹^,², Jason Jerwick²^,³, Penghe Wu¹^,², Mingming Chen³^,⁴, Aneesh Alex²^,³, Yutao Ma⁴, Rudolph E. Tanzi⁵, Airong Li⁵, Chao Zhou¹^,²^,³

¹Bioengineering Program, Lehigh University, ²Center for Photonics and Nanoelectronics, Lehigh University, ³Department of Electrical and Computer Engineering, Lehigh University, ⁴State Key Laboratory of Software Engineering, Wuhan University, ⁵Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School

Summary

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Keywords Drosophila

Heart Function

Optical Coherence Microscopy OCM

Chapters in this video

0:05

Title

1:08

Mounting Larva for OCM Imaging

2:13

Imaging Larval Hearts with OCM

4:39

Imaging at the PD1 Stage

5:52

Imaging at the PD2 to PD5 Stages

7:25

Imaging at the Adult Stage

8:34

Results: Heart Rate during Development

9:36

Conclusion

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