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The ChIP-exo Method: Identifying Protein-DNA Interactions with Near Base Pair Precision

16.4K Views

09:27 min

December 23rd, 2016

December 23rd, 2016

16,384 Views

0:05

Title

0:41

Sonication of Nuclear Lysates

2:31

Chromatin Immunoprecipitation

3:55

P7 Adapter Ligation and Lambda Exonuclease Reactions

5:06

RecJf Nuclease, Crosslink Reversal, and P5 Adapter Ligation Reactions

6:47

Results: Chromatin Immunoprecipitation Exo-mediated Protein-DNA Interactions

8:21

Conclusion

Transcript

The overall goal of this functional genomic technology is to identify the precise genomic locations that a given protein occupies in living cells. This method can help answer key questions in the fields of gene regulation and epigenetics such as h

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Here, we present a protocol to achieve near base pair resolution of protein-DNA interactions. This is obtained by exonuclease treatment of DNA fragments selectively enriched by chromatin immunoprecipitation (ChIP-exo) followed by high throughput sequencing.

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