JoVE Logo
Faculty Resource Center

Sign In

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

DOI :

10.3791/55836-v

June 3rd, 2017

June 3rd, 2017

10,782 Views

1Basic Sciences Division, Fred Hutchinson Cancer Research Center, 2Department of Biology, Indiana University

We describe chromatin endogenous cleavage coupled with high-throughput sequencing (ChEC-seq), a chromatin immunoprecipitation (ChIP)-orthogonal method for mapping protein binding sites genome-wide with micrococcal nuclease (MNase) fusion proteins.

Tags

Genome wide Mapping

-- Views

Related Videos

article

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

article

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

article

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

article

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

article

Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos

article

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

article

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

article

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

article

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

article

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved