Collecting Cryosections for RNA Extraction and Histology
4:20
RNA Isolation from Pooled Cryosections
6:17
Results: RNA Extraction and Histology of Cardiotoxin Injured Muscle
7:40
Conclusion
Transcript
The overall goal of this procedure is to enable analysis of histological and molecular parameters from adjacent sections of a single tissue. With this method, we can help to answer key questions in the field skeletal muscle biology and disease. Su
Sign in or start your free trial to access this content
Consecutive cryo-sections are collected to enable histological applications and enrichment of RNA for gene expression measurements using adjacent regions from a single mouse skeletal muscle. High-quality RNA is obtained from 20 - 30 mg of pooled cryosections and measurements are directly compared across applications.