Collecting Cryosections for RNA Extraction and Histology
4:20
RNA Isolation from Pooled Cryosections
6:17
Results: RNA Extraction and Histology of Cardiotoxin Injured Muscle
7:40
Conclusion
필기록
The overall goal of this procedure is to enable analysis of histological and molecular parameters from adjacent sections of a single tissue. With this method, we can help to answer key questions in the field skeletal muscle biology and disease. Su
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Consecutive cryo-sections are collected to enable histological applications and enrichment of RNA for gene expression measurements using adjacent regions from a single mouse skeletal muscle. High-quality RNA is obtained from 20 - 30 mg of pooled cryosections and measurements are directly compared across applications.