December 12th, 2016
•Consecutive cryo-sections are collected to enable histological applications and enrichment of RNA for gene expression measurements using adjacent regions from a single mouse skeletal muscle. High-quality RNA is obtained from 20 - 30 mg of pooled cryosections and measurements are directly compared across applications.
Related Videos
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
In vitro Reconstitution of the Active T. castaneum Telomerase
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
Tissue Triage and Freezing for Models of Skeletal Muscle Disease
Measurement of Metabolic Rate in Drosophila using Respirometry
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved