Purification and Expansion of Murine Primary NK Cells
2:03
Purification and Expansion of Human Primary NK Cells
3:23
Transduction of Murine and Human Primary NK Cells with Lentivirus
6:25
Results: Lentiviral Transduction Efficiency Enhanced with Dextran
7:58
Conclusion
Transcript
The overall goal of this protocol is to formulate technologies that allow successful gene transduction in primary natural killer cells. This method can help answer key questions in the Immunotherapy field, like the effect of genetic modification o
Sign in or start your free trial to access this content
The goal of this study was to formulate technologies that allow for successful gene transduction in primary natural killer (NK) cells. The dextran-mediated lentiviral transduction of human or mouse primary NK cells results in higher gene expression efficiencies. This method of gene transduction will vastly improve NK cell genetic manipulation.