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Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation

DOI :

10.3791/2766-v

July 8th, 2011

July 8th, 2011

20,057 Views

1Center for Translational Systems Biology and Department of Neurology, Mount Sinai School of Medicine

We present our optimized high-throughput nucleofection protocol as an efficient way of transfecting primary human monocyte-derived dendritic cells with either plasmid DNA or siRNA without causing cell maturation. We further provide evidence for successful siRNA silencing of targeted gene RIG-I at both the mRNA and protein levels.

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