Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
10.9K Views
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08:42 min
•
January 15th, 2018
DOI :
January 15th, 2018
•Arash Nanbakhsh1, Brad Best2, Matthew Riese3, Sridhar Rao4, Li Wang5, Jeffrey Medin6, Monica S. Thakar6, Subramaniam Malarkannan1,5,6,7
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, 5Department of Microbiology and Immunology, The Medical College of Wisconsin, 6Department of Pediatrics, The Medical College of Wisconsin, 7Department of Medicine, The Medical College of Wisconsin
Chapters in this video
0:05
Title
0:44
Purification and Expansion of Murine Primary NK Cells
2:03
Purification and Expansion of Human Primary NK Cells
3:23
Transduction of Murine and Human Primary NK Cells with Lentivirus
6:25
Results: Lentiviral Transduction Efficiency Enhanced with Dextran
7:58
Conclusion
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