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Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

DOI :

10.3791/58081-v

October 30th, 2018

October 30th, 2018

9,810 Views

1Laboratory of Leukocyte Signalling, Institute of Molecular Genetics of the ASCR, 2Faculty of Science, Charles University, 3Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry ASCR

In this article, we provide a detailed protocol for the expression of fluorescent fusion proteins in murine bone marrow derived dendritic cells and macrophages. The method is based on the transduction of bone marrow progenitors with retroviral constructs followed by differentiation into macrophages and dendritic cells in vitro.

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