Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
10.6K Views
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09:45 min
•
January 1st, 2017
DOI :
January 1st, 2017
•Wei Wen1, Jian-Ping Zhang1, Wanqiu Chen2, Cameron Arakaki2, Xiaolan Li1,2, David Baylink2, Gary D. Botimer3, Jing Xu1, Weiping Yuan1, Tao Cheng1,4,5,6,7, Xiao-Bing Zhang1,2
1State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 2Division of Regenerative Medicine, Department of Medicine, Loma Linda University, 3Department of Orthopaedic Surgery, Loma Linda University, 4Center for Stem Cell Medicine, Chinese Academy of Medical Sciences, 5Department of Stem Cell & Regenerative Medicine, Peking Union Medical College, 6Collaborative Innovation Center for Cancer Medicine, 7Tianjin Key Laboratory of Blood Cell Therapy and Technology
Chapters in this video
0:05
Title
1:01
Isolation of Peripheral Blood Mononuclear Cells (PB MNCs)
3:03
Expansion of PB MNCs in Erythroid Medium
4:07
PB MNCs Nucleofection and Reprogramming
8:00
Results: Generation of Integration-free iPSCs from PB MNCs
8:48
Conclusion
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