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Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

DOI :

10.3791/53174-v

August 20th, 2015

August 20th, 2015

11,168 Views

1Centre for Eye Research Australia & Department of Ophthalmology, University of Melbourne

This manuscript provides a step-by-step procedure for the derivation and maintenance of human keratinocytes from plucked hair and subsequent generation of integration-free human induced pluripotent stem cells (hiPSCs) by episomal vectors.

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Integration free Human Induced Pluripotent Stem Cells

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