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Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

6.1K Views

11:06 min

September 20th, 2017

DOI :

10.3791/55135-v

September 20th, 2017


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Genome wide Analysis

Chapters in this video

0:05

Title

0:56

Stimulation of Mouse B Cells for Class-switch DNA Recombination (CSR), Plasma Differentiation and HDI Treatment

3:04

B-cell Isolation

4:26

B-cell Stimulation and HDI Treatment

5:17

Flow Cytometry Analysis of CSR and Plasma Cell Differentiation

7:11

Quantitative RT-PCR of mRNA and miRNAs

8:41

Results: Analysis of Epigenetic Modulation of miRNA and Gene Expression in B Cells

10:28

Conclusion

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