JoVE Logo
Faculty Resource Center

Sign In

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

DOI :

10.3791/2130-v

August 13th, 2010

August 13th, 2010

17,830 Views

1Department of Immunology, University of Toronto

Following antigen exposure, subpopulations of activated B cells undergo a process known as class switch recombination (CSR) to produce antibody isotypes with distinct effector functions. The protocol outlined in this report explains how CSR can be induced and analyzed in vitro for the purposes of studying B cell function.

-- Views

Related Videos

article

Murine Model of CD40-activation of B cells

article

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

article

Detection of True IgE-expressing Mouse B Lineage Cells

article

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

article

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

article

A Murine Model of Group B Streptococcus Vaginal Colonization

article

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

article

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

article

Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro

article

Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved