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Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

DOI :

10.3791/2101-v

11:19 min

July 7th, 2010

July 7th, 2010

14,146 Views

1Department of Biochemistry and Molecular Genetics, University of Illinois Chicago - UIC, 2Research Unit on Biomedical Informatics, Universitat Pompeu Fabra, 3Genome Technology Core, Whitehead Institute for Biomedical Research

Here we are presenting a chromatin immunoprecipitation (ChIP) procedure for genome-wide location analysis of protein isoforms that differ in a histone-binding domain. We are applying it to ChIP-Seq analysis to identify the targets of the KDM5A/JARID1A/RBP2 histone demethylase.

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Genome wide Analysis

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