Recombinant Retroviral Production and Infection of B Cells

14.0K Views

09:19 min

February 18th, 2011

DOI :

10.3791/2371-v

February 18th, 2011


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Recombinant Retroviral Production

Chapters in this video

0:05

Title

1:24

Splenic B-Lymphocyte Isolation and Stimulation

5:42

Transfection of Producer Cells

7:26

Infection of Stimulated Splenic B cells

8:12

Flow Cytometry Analysis

8:40

Rescue of AID-Deficiency by Transduction of AID-Deficient B Cells with a Recombinant Retrovirus

8:57

Conclusion

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