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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

DOI :

10.3791/55196-v

November 1st, 2017

November 1st, 2017

10,507 Views

1Department of Internal Medicine, Hematology Division, Washington University in St. Louis

Here, we present human pluripotent stem cell (hPSC) culture protocols, used to differentiate hPSCs into CD34+ hematopoietic progenitors. This method uses stage-specific manipulation of canonical WNT signaling to specify cells exclusively to either the definitive or primitive hematopoietic program.

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Directed Differentiation

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