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Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

DOI :

10.3791/55212-v

8:13 min

December 27th, 2016

December 27th, 2016

18,103 Views

1Department of Neuroscience, Cell Biology and Physiology, Boonshoft School of Medicine, Wright State University, 2Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University

T-lymphocyte mitogenesis is accompanied by blastogenic transformation, whereupon the cell volume enlarges before cell division. Here, we describe a method to quantify blastogenesis in T lymphocytes using an automated cell counter with the capability of measuring cell diameters.

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Rapid Quantification

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