December 28th, 2011
•Single cell expression profiling allows the detailed gene expression analysis of individual cells. We describe methods for the isolation of cardiomyocytes, and preparing the resulting lysates for either whole transcriptome microarray or qPCR of specific targets.
Tags
Related Videos
Generation of Single-Cell Suspensions from Mouse Neural Tissue
Isolation and Culture of Neonatal Mouse Cardiomyocytes
Isolation and Physiological Analysis of Mouse Cardiomyocytes
Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy
Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types
Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes
Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
Cell Type-specific Gene Expression Profiling in the Mouse Liver
Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved