Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation
12.3K Views
•
08:05 min
•
July 3rd, 2017
DOI :
July 3rd, 2017
•Rupesh Agrawal*1,2,3, Praveen Kumar Balne*2, Sai Bo Bo Tun2, Yeo Sia Wey2, Neha Khandelwal1, Veluchamy A. Barathi2,4,5
1National Healthcare Group Eye Institute, Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), Singapore National Eye Center, 3School of Material Science and Engineering, Nanyang Technological University, 4Department of Ophthalmology, Yong Loo Lin School of Medicine, National University Health Systems, National University of Singapore, 5Ophthalmology Academic Clinical Research Program, DUKE-NUS Graduate Medical School
Chapters in this video
0:05
Title
0:54
Isolation and Labeling of Erythrocytes (ICG) and Leukocytes (Sodium Fluorescein)
3:25
Live-cell Imaging of Labeled Erythrocytes
5:26
Image Analysis Using MtrackJ Software
6:56
Results: Visualizaing the Labeled Erythrocytes and Leukocytes
7:38
Conclusion
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