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Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy

DOI :

10.3791/55514-v

April 9th, 2017

April 9th, 2017

10,403 Views

1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University, 2Institute of Biochemistry, College of Life Sciences, Zhejiang University

This protocol describes confocal microscopy detection of G protein-coupled receptor (GPCR) internalization in mammalian cells. It includes the basic cell culture, transfection, and confocal microscopy procedure and provides an efficient and easily interpretable method to detect the subcellular localization and internalization of fusion-expressed GPCR.

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Ligand activated

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