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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors Video (Video) | JoVE

DOI :

10.3791/50588-v

September 13th, 2013

September 13th, 2013

15,013 Views

1Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University

We genetically-encode the unnatural amino acid, p-azido-L-phenylalanine at various targeted positions in GPCRs and show the versatility of the azido group in different applications. These include a targeted photocrosslinking technology to identify residues in the ligand-binding pocket of a GPCR, and site-specific bioorthogonal modification of GPCRs with a peptide-epitope tag or fluorescent probe.

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