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Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

DOI :

10.3791/61060-v

June 8th, 2020

June 8th, 2020

11,306 Views

1NCI CCR Sequencing Facility, Frederick National Laboratory for Cancer Research, 2Advanced Biomedical and Computational Sciences, Frederick National Laboratory for Cancer Research

This method describes the steps to improve the quality and quantity of sequence data that can be obtained from formalin-fixed paraffin-embedded (FFPE) RNA samples. We describe the methodology to more accurately assess the quality of FFPE-RNA samples, prepare sequencing libraries, and analyze the data from FFPE-RNA samples.

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