June 8th, 2020
•This method describes the steps to improve the quality and quantity of sequence data that can be obtained from formalin-fixed paraffin-embedded (FFPE) RNA samples. We describe the methodology to more accurately assess the quality of FFPE-RNA samples, prepare sequencing libraries, and analyze the data from FFPE-RNA samples.
Tags
Related Videos
Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
Detection of Copy Number Alterations Using Single Cell Sequencing
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved