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Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

DOI :

10.3791/55463-v

March 31st, 2017

March 31st, 2017

11,289 Views

1Department of Plant and Microbial Biology, University of California Berkeley, 2John Innes Centre, Norwich Research Park, 3Department of Plant and Microbial Biology, University of Zürich, 4USDA-ARS Sunflower and Plant Biology Research Unit

Described here is a method for the extraction, purification, and quality control of genomic DNA from the obligate biotrophic fungal pathogen, powdery mildew, for use in long-read genome sequencing.

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