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Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

7.4K Views

12:23 min

May 16th, 2017

DOI :

10.3791/55576-v

May 16th, 2017


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Keywords Recombinant Protein

Chapters in this video

0:05

Title

0:55

Recombinant Protein Production and Purification of rBC1531

4:47

Removal of Affinity Tags by Thrombin Proteolysis

6:32

Crystallization Screening and Optimization of rBC1531

7:30

Colorimetric NTPase Assay

8:33

Results: Purification, Characterization, and Crystallization of rBC1531

10:31

Conclusion

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