Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

19.0K Views

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12:00 min

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July 5th, 2017

DOI :

10.3791/55605-v

July 5th, 2017

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Rafaela G. Feresin¹, Shirin Pourafshar²^,³, Jingwen Huang², Yitong Zhao², Bahram H. Arjmandi²^,³, Gloria Salazar²^,³

¹Department of Dietetics and Nutrition, University of Arkansas for Medical Sciences, ²Department of Nutrition, Food and Exercise Sciences, Florida State University, ³Center for Advancing Exercise and Nutrition Research on Aging (CAENRA), Florida State University

Summary

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Keywords Polyphenol Extraction

Freeze dried Berry Powder

Ultrasonic assisted Extraction

Ethanol based Extraction

Chloroform Purification

Ethyl Acetate Fractionation

Vascular Smooth Muscle Cells

In Vitro Treatment

Chapters in this video

0:05

Title

1:08

Preparation of Crude Blackberry Extracts

3:31

Preparation of Polyphenol Extracts from Crude Berry Extracts

5:13

Treatment of Vascular Smooth Muscle Cells with Berry Extracts-purified Polyphenols

9:08

Results: Polyphenol Composition and Phosphorylation Reduction by Crude and Purified Berry Extracts

11:05

Conclusion

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