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Isolation of Murine Coronary Vascular Smooth Muscle Cells

DOI :

10.3791/53983-v

May 30th, 2016

May 30th, 2016

13,380 Views

1School of Biomedical Science, The Ohio State University College of Medicine, 2Center for Cardiovascular and Pulmonary Research, The Research Institute at Nationwide Children's Hospital, 3Department of Pediatrics, The Ohio State University College of Medicine

The purpose of this protocol is to demonstrate the isolation and culture techniques of murine primary vascular smooth muscle cells (VSMCs) from the coronary circulation. Once VSMCs have been isolated, they can be used for many standard culture techniques.

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