JoVE Logo
Faculty Resource Center

Sign In

Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

DOI :

10.3791/63999-v

9:00 min

June 10th, 2022

June 10th, 2022

1,969 Views

1Department of Kinesiology and Applied Physiology, College of Health Sciences, University of Delaware, 2Division of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, 3University of Delaware Flow Cytometry Core, Delaware Biotechnology Institute, University of Delaware

This protocol details a method for the dissection of mouse adipose depots and the isolation and digestion of respective arteries to liberate and then identify the endothelial cell population. Freshly isolated cells used in downstream applications will advance the understanding of vascular cell biology and the mechanisms of vascular dysfunction.

Tags

Isolation

-- Views

Related Videos

article

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

article

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

article

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

article

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

article

A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods

article

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

article

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

article

Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

article

Generation of Patient-Derived Podocytes from Skin Biopsies

article

Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved