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A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

DOI :

10.3791/55616-v

April 6th, 2017

April 6th, 2017

10,908 Views

1State Key Laboratory for Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University

This protocol describes a procedure to freeze muscle tissues by plunging them directly into liquid nitrogen. This protocol also highlights a new cryovial that can avoid the "blanket effect" of nitrogen gas when liquid nitrogen contacts the tissue surface of a specimen.

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Multi hole Cryovial

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