Tissue Triage and Freezing for Models of Skeletal Muscle Disease

39.8K Views

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05:58 min

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July 15th, 2014

DOI :

10.3791/51586-v

July 15th, 2014

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Hui Meng¹, Paul M.L. Janssen², Robert W. Grange³, Lin Yang⁴, Alan H. Beggs⁵, Lindsay C. Swanson⁵, Stacy A. Cossette¹^,⁶, Alison Frase⁷, Martin K. Childers⁸, Henk Granzier⁹, Emanuela Gussoni⁵, Michael W. Lawlor¹

¹Division of Pediatric Pathology, Department of Pathology and Laboratory Medicine, Medical College of Wisconsin, ²Department of Physiology and Cell Biology, The Ohio State University, ³Department of Human Nutrition, Foods and Exercise, Virginia Tech, ⁴Division of Biomedical Informatics, Department of Biostatistics, Department of Computer Science, University of Kentucky, ⁵Division of Genetics and Genomics, The Manton Center for Orphan Disease Research, Boston Children's Hospital, Harvard Medical School, ⁶Cure Congenital Muscular Dystrophy, ⁷Joshua Frase Foundation, ⁸Department of Rehabilitation Medicine, University of Washington, ⁹Department of Physiology, University of Arizona

Summary

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Congenital Muscle Disorder

Histological Examination

Isopentane

Liquid Nitrogen

Single Fiber Functional Studies

Myoblast Cell Culture

Phenotypic Studies

Standard Operating Procedures

Chapters in this video

0:05

Title

1:18

Tissue Processing

4:24

Results: Examples of Freezing Artifacts and Effective Thawing/Refreezing

5:35

Conclusion

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