Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
19.8K Views
•
08:03 min
•
June 25th, 2017
DOI :
June 25th, 2017
•Stephanie Chanon1, Christine Durand1, Aurelie Vieille-Marchiset1, Maud Robert2, Charna Dibner3, Chantal Simon1, Etienne Lefai1
1CarMeN Laboratory, INSERM U1060, INRA 1397, University of Lyon, 2Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon, Lyon 1 University, 3Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine, University of Geneva
Chapters in this video
0:05
Title
0:34
Cell Culture Media and Solution Preparation
2:45
Culture of Human Primary Muscle Cells
4:19
Insulin Stimulation and Glucose Uptake
5:30
Cell Lysis
6:06
Determination of Radiolabeled Glucose
6:52
Results: Effective Glucose Uptake Measurement and Analysis of Response to Insulin Stimulation
7:37
Conclusion
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