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Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

19.5K Views

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08:03 min

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June 25th, 2017

DOI :

10.3791/55743-v

June 25th, 2017

•

19,499 Views

Stephanie Chanon¹, Christine Durand¹, Aurelie Vieille-Marchiset¹, Maud Robert², Charna Dibner³, Chantal Simon¹, Etienne Lefai¹

¹CarMeN Laboratory, INSERM U1060, INRA 1397, University of Lyon, ²Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon, Lyon 1 University, ³Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine, University of Geneva

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Glucose Uptake

Insulin Stimulation

Human Primary Myotubes

Muscle Metabolism

Insulin Sensitivity

Proliferation Medium

Differentiation Medium

2DG

Cytochalasin B

DMSO

Muscle Satellite Cells

Cell Culture

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