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Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

DOI :

10.3791/55756-v

April 20th, 2017

April 20th, 2017

10,446 Views

1U.S. Arid Land Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture

Nonlytic insect cell expression systems are underutilized for production, cellular trafficking/localization, and recombinant protein functional analysis. Here, we describe methods to generate expression vectors and subsequent transient protein expression in commercially available lepidopteran cell lines. The co-localization of Bemisia tabaci aquaporins with subcellular fluorescent marker proteins is also presented.

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Keywords Insect Cells

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