Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells

6.7K Views

•

11:00 min

•

July 6th, 2019

DOI :

10.3791/59661-v

July 6th, 2019

•

Shaoyang Wang¹^,², Minglie Hu², Tao Ren¹, Hao He³

¹Department of Respiratory Medicine, Shanghai Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University, ²Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, ³School of Biomedical Engineering, Shanghai Jiao Tong University

Summary

Explore More Videos

Single cell Operation

Cellular Signaling

HeLa Cells

Fluorescence Microscopy

Optical Manipulation

Laser Scanning

Molecular Dynamics

Experimental Protocol

Chapters in this video

0:04

Title

1:04

Setting Up the Photostimulation System

1:48

Preparing for Activation of ERK2 by Photostimulation

2:47

Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-A Mode

4:24

Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-B Mode

5:53

Activation of Mitoflashes and Other Mitochondrial Events by Photostimulation

6:55

Delivering Femtosecond Laser Stimulation into the Target Mitochondrion Using Stim-A Mode

8:32

Results: Activation of ERK Signaling or Mitochondrial Events in Target Cells

10:26

Conclusion

Related Videos

09:40

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum

17.9K Views

10:59

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)

16.1K Views

10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

23.0K Views

15:43

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes

18.1K Views

12:06

Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo

14.5K Views

12:54

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

13.8K Views

08:24

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

10.0K Views

09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

8.5K Views

08:43

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

18.8K Views

08:12

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

7.3K Views