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Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells

DOI :

10.3791/55842-v

May 15th, 2017

May 15th, 2017

9,081 Views

1Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology

This protocol enables single cell microscopy of the differentially distinct Vibrio parahaemolyticus swimmer and swarmer cells. The method produces a population of swarmer cells easily available for single cell analysis and covers preparation of cell cultures, induction of swarmer differentiation, sample preparation, and image analysis.

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