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Colonization of Euprymna scolopes Squid by Vibrio fischeri

DOI :

10.3791/3758-v

March 1st, 2012

March 1st, 2012

19,542 Views

1Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University

The method outlines the procedure by which the Hawaiian bobtail squid, Euprymna scolopes and its bacterial symbiont, Vibrio fischeri, are raised separately and then introduced to allow for specific colonization of the squid light organ by the bacteria. Colonization detection by bacterially-derived luminescence and by direct colony counting are described.

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Colonization

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