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Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

DOI :

10.3791/55997-v

June 24th, 2017

June 24th, 2017

7,208 Views

1Laboratory of Cellular & Molecular Biology, National Cancer Institute, National Institutes of Health, 2Laboratory of Single Molecule Biophysics, National Heart, Lung, and Blood, Institute, National Institutes of Health

We demonstrate a method to image multiple molecules within heterogeneous nano-structures at single molecule accuracy using sequential binding and elution of fluorescently labeled antibodies.

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Keywords T Cells

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