Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
12.8K Views
•
11:29 min
•
July 10th, 2017
DOI :
July 10th, 2017
•Frank J. Jenkins1,2, Charles M. Kerr2, Elise Fouquerel2,3, Dana H. Bovbjerg2,4, Patricia L. Opresko2,3
1Departments of Pathology and Infectious Diseases and Microbiology, University of Pittsburgh, 2University of Pittsburgh Cancer Institute, 3Department of Environmental and Occupational Health, University of Pittsburgh, 4Departments of Psychiatry, Psychology, Behavioral & Community Health Sciences, University of Pittsburgh
Chapters in this video
0:05
Title
0:46
Genomic DNA Digestion
1:39
Genomic DNA Gel Electrophoresis
2:56
Gel Fluorescent Imaging and Hybridization
5:17
Hybridization with Telomere Probe
7:36
Gel Washing, Phospher Screen Exposure, and Radio Imaging
9:08
Results: Telomere Length is Quantified by a Modefied Terminal Restriction Fragment Analysis
10:28
Conclusion
Related Videos
15:25
Examination of the Telomere G-overhang Structure in Trypanosoma brucei
14.2K Views
08:57
Laparoscopic Pancreatoduodenectomy With Modified Blumgart Pancreaticojejunostomy
14.8K Views
11:20
Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
10.8K Views
09:13
Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
7.3K Views
06:38
Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
8.7K Views
11:44
Monochrome Multiplex Quantitative PCR Telomere Length Measurement
1.4K Views
08:23
Optimization of Performance Parameters of the TAGGG Telomere Length Assay
2.6K Views
11:21
Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers
589 Views
12:08
Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
46.4K Views
10:01
Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans
10.3K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved