JoVE Logo
Faculty Resource Center

Sign In

Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells

DOI :

10.3791/58790-v

January 17th, 2019

January 17th, 2019

7,208 Views

1Laboratory of Cell Biology and Molecular Genetics, Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, 2Department of Life Sciences, University of Trieste

Here, we present a protocol to generate cancer cell clones containing a MS2 sequence tag at a single subtelomere. This approach, relying on the MS2-GFP system, enables visualization of the endogenous transcripts of telomeric repeat-containing RNA (TERRA) expressed from a single telomere in living cells.

Tags

Cancer Cell Clones

-- Views

Related Videos

article

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

article

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

article

Detection of Copy Number Alterations Using Single Cell Sequencing

article

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

article

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

article

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

article

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

article

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

article

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

article

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved