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Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction

DOI :

10.3791/59550-v

May 3rd, 2019

May 3rd, 2019

8,446 Views

1School of Kinesiology, University of Michigan

We successfully converted the standard telomere repeat amplification protocol (TRAP) assay to be employed in droplet digital polymerase chain reactions. This new assay, called ddTRAP, is more sensitive and quantitative, allowing for better detection and statistical analysis of telomerase activity within various human cells.

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Keywords DdTRAP

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