May 3rd, 2019
•We successfully converted the standard telomere repeat amplification protocol (TRAP) assay to be employed in droplet digital polymerase chain reactions. This new assay, called ddTRAP, is more sensitive and quantitative, allowing for better detection and statistical analysis of telomerase activity within various human cells.
Related Videos
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
Counting Proteins in Single Cells with Addressable Droplet Microarrays
Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR
Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues
Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis
Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
Pathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells
Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved