JoVE Logo
Faculty Resource Center

Sign In

Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets

DOI :

10.3791/56089-v

September 13th, 2017

September 13th, 2017

8,887 Views

1Department of Medicine, University of Miami, 2Department of Medicine, University of Toronto

We describe a protocol for visualization of insulin exocytosis in intact islets using pHluorin, a pH-sensitive green fluorescent protein. Isolated islets are infected with adenovirus encoding pHluorin coupled to the vesicle cargo neuropeptide Y. This allows for the detection of insulin granule fusion events by confocal microscopy.

Tags

Confocal Imaging

-- Views

Related Videos

article

Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy

article

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

article

A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ

article

Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster

article

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

article

Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo

article

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

article

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

article

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

article

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved