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Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

DOI :

10.3791/52632-v

May 11th, 2015

May 11th, 2015

9,203 Views

1School of Biomedical Sciences, The University of Queensland

We present here a protocol for the isolation of islets from the mouse model of type 2 diabetes, Leprdb and details of a live-cell assay for measurement of insulin secretion from intact islets that utilizes 2 photon microscopy.

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Type 2 Diabetes

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